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目的:研究六经头痛片以提高其质量标准。方法:采用薄层色谱法对制剂中白芷、葛根进行定性鉴别;采用高效液相色谱法测定制剂中葛根素含量,岛津C18柱(4.6 mm×250 mm,5μm),流速1 mL.min-1,流动相甲醇-0.025 mol.L-1磷酸溶液(20∶80),柱温30℃,检测波长250 nm。结果:在薄层色谱中均可检出白芷、葛根的特征斑点;葛根素在0.042～0.378μg线性关系良好(r=0.999 9),回收率98.368%(RSD 1.87%)。结论:方法简便可行、重复性好,能有效控制该制剂的质量。
OBJECTIVE: To study Liujing headache tablets to improve its quality standards. Methods: The Angelica dahurica and Radix Puerariae were identified by TLC. The content of puerarin in the preparation was determined by HPLC. Shimadzu C18 column (4.6 mm × 250 mm, 5 μm) was used with a flow rate of 1 mL · min- 1, the mobile phase methanol -0.025 mol.L-1 phosphoric acid solution (20:80), the column temperature 30 ℃, detection wavelength 250 nm. Results: The characteristic spots of Angelica dahurica and Radix Puerariae were detected by TLC. The linearity of puerarin was 0.042 ~ 0.378 μg (r = 0.999 9), the recovery was 98.368% (RSD 1.87%). Conclusion: The method is simple and feasible, reproducible, and can effectively control the quality of the preparation.