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该项目在国内首次建立了人S-100β重组构建、体外表达载体的克隆、体外表达、获得重组人S-100β,并建立了人S-100βmRNA的分析方法和S-100PCR—SSCP突变分析方法;首次分析不同类型肺癌组织S—100βmRNA的表达情况。结果表明小细胞肺癌细胞及其周边组织某些细胞S-100β表达增加。而腺癌、鳞癌细胞无S-100β表达和明
The project was the first to establish a human S-100β recombinantly constructed, in vitro expression vector cloning, expression in vitro, to obtain recombinant human S-100β, and established human S-100β mRNA analysis and S-100PCR-SSCP mutation analysis; For the first time analysis of different types of lung cancer S-100βmRNA expression. The results showed that small cell lung cancer cells and their surrounding tissues S-100β expression increased in some cells. The adenocarcinoma, squamous cell carcinoma without S-100β expression and Ming