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目的 :采用RP HPLC法测定四物膏中芍药苷的含量。方法 :用KromasilC18柱 ,以甲醇 水 ( 2 1∶79)为流动相 ;检测波长 :2 30nm ;温度 30℃。结果 :芍药苷在 0 .2 0 32～ 1.0 16 μg内呈良好线性关系 ,r =0 .9998,平均加样回收率为 10 0 .4 % ,RSD =1.4 % (n =5 )。结论 :方法简便 ,快速 ,分离度好 ,结果稳定 ,可用于四物膏的质量控制
Objective : To determine the content of paeoniflorin in Siwu cream by RP HPLC. Method : Kromasil C18 column with methanol water (2 1∶79) as mobile phase; detection wavelength: 2 30nm; temperature 30°C. Results: There was a good linear relationship between paeoniflorin ranged from 0.232 to 1.016 μg, r = 0.998, the average recovery was 100.4%, RSD = 1.4% (n = 5). Conclusion : The method is simple, rapid, and has good resolution and stable results. It can be used for the quality control of Siwu cream.