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目的：研究８－甲氧基补骨脂素（８－ＭＯＰ）对粘液表皮样癌细胞（ＭＥＣ－１）的抑制作用。方法：应用常规ＭＴＴ法、细胞计数法和流式细胞术研究８－ＭＯＰ作用于粘液表皮样癌细胞的剂量－效应关系和时间－效应关系以及３０％抑制浓度的药物对细胞群体倍增时间和细胞周期的影响。结果：１）小剂量８－ＭＯＰ在短时间内对ＭＥＣ－１细胞没有抑制作用，当剂量大于２５ｍｇ／Ｌ时，药物的抑制作用呈剂量依赖性；作用时间在２４ｈ～７２ｈ之间时表现出时间依赖性关系；药物的抑制作用有延迟性。２）１２０ｈ作用组３０％抑制浓度ＩＣ３０＝３３．８６ｍｇ／Ｌ，５０％抑制浓度ＩＣ５０＝４５．３ｍｇ／Ｌ，ＲＡＡ值＝２．９。３）它阻止细胞进入Ｓ期完成细胞分裂。结论：ＭＥＣ－１细胞对８－ＭＯＰ较敏感，８－ＭＯＰ在人粘液表皮样癌的防治方面可能有一定意义。
Objective: To study the inhibitory effect of 8-methoxy psoralen (8-MOP) on mucoepidermoid carcinoma cells (MEC-1). Methods: The dose-effect relationship and time-effect relationship of 8-MOP on mucoepidermoid carcinoma cells were studied by conventional MTT assay, cytometry and flow cytometry. The effect of 30% inhibitory concentration on the doubling time and cell population The impact of the cycle. The results showed that: 1) The low dose of 8-MOP had no inhibitory effect on MEC-1 cells in a short period of time. When the dosage was more than 25mg / L, the inhibition of drug was dose-dependent; the effect time was between 24h and 72h Time-dependent relationship; drug inhibition is delayed. 2) The 30% inhibitory concentration (IC30 = 33.86 mg / L) at 120 h and the IC50 at 50% inhibition were 45.3 mg / L and RAA = 2.9.3) It prevented the cells from entering S phase to complete cell division. Conclusion: MEC-1 cells are more sensitive to 8-MOP, 8-MOP may have some significance in the prevention and treatment of human mucoepidermoid carcinoma.