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为了探究几种骨组织细胞系的力学性能及其与细胞功能的关系,该文采用原子力显微镜压陷法分别检测了前成骨细胞系(2T3和MC3T3-E1)、前骨细胞系(MLO-A5)和骨样细胞系(MLO-Y4)的杨氏模量,利用激光共聚焦显微镜观察了这几种细胞微丝和微管的排布。结果显示,2T3,MC3T3-E1、MLO-A5和MLO-Y4细胞的杨氏模量分别为(7000±2015)Pa、(6600±2024)Pa、(4700±1644)Pa和(4500±1622)Pa,与原代骨组织细胞的杨氏模量及变化趋势保持一致,但两种前成骨细胞的杨氏模量要显著高于前骨细胞和骨细胞。细胞荧光染色结果表明,前成骨细胞细胞核周围的微丝和微管分布密度要高于前骨细胞和骨细胞,而前骨细胞MLO-A5,尤其是骨细胞MLOY4的骨架主要集中于细胞突触和边缘,这可能是导致几种细胞力学性能差异的原因。该研究从生物力学的角度为进一步深入理解骨组织细胞结构与功能的关系提供了实验依据。
In order to investigate the mechanical properties of several bone tissue cell lines and their relationship with cell function, we used atomic force microscopy (AFM) to detect the expressions of the pre-osteoblast lines (2T3 and MC3T3-E1), the pre-osteoblast line (MLO- A5) and osteoblast-like cell line (MLO-Y4). The arrangement of microfilaments and microtubules was observed by laser confocal microscopy. The results showed that the Young’s Modulus of 2T3, MC3T3-E1, MLO-A5 and MLO-Y4 cells were (7000 ± 2015) Pa, (6600 ± 2024) Pa, (4700 ± 1644) Pa and (4500 ± 1622) Pa, which were consistent with the Young’s modulus and trend of primary bone cells, but Young’s modulus of both pre-osteoblasts was significantly higher than that of pre-osteocytes and osteocytes. Fluorescent staining showed that the distribution density of microfilaments and microtubules around the nucleus of pre-osteoblasts was higher than that of pre-osteocytes and osteocytes, while the skeleton of MLO-A5, especially osteoblast MLOY4, was predominantly located in the nucleus Touch and edge, which may be the reason for the differences in the mechanical properties of several cells. This study provides experimental evidence for further understanding of the relationship between cell structure and function of bone tissue from the biomechanical point of view.