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目的观察ＨＢｃＡｇＤＮＡ疫苗（ｐＪＷ４３０３／ＨＢｃ）免疫Ｃ５７ＢＬ／６小鼠（Ｈ２ｂ）的特异性细胞和体液免疫应答。方法基因枪和肌肉注射两种方法接种ＤＮＡ疫苗；ＥＬＩＳＡ法检测小鼠血清抗ＨＢｃ（ＩｇＧ）及ＩｇＧ亚类（ＩｇＧ１，ＩｇＧ２ａ）；５１铬释放法检测小鼠脾细胞ＨＢｃＡｇ特异性ＣＴＬ活性。结果该ＤＮＡ疫苗体外可表达ＨＢｃＡｇ；小鼠经基因枪或肌肉注射接种该疫苗后血清抗ＨＢｃ滴度分别达１∶３２８０５０和１∶１０９３５０；肌肉注射组抗ＨＢｃ亚类以ＩｇＧ２ａ为主；两种方法接种小鼠的特异性ＣＴＬ杀伤率分别达５３．１％和５２．８％。结论ＨＢｃＡｇＤＮＡ疫苗在Ｈ２ｂ小鼠实验中表现出良好的细胞免疫和体液免疫原性。
Objective To observe the specific cellular and humoral immune responses of C57BL / 6 mice (H2b) immunized with HBcAg DNA vaccine (pJW4303 / HBc). Methods DNA vaccines were administered by gene gun and intramuscular injection. Serum anti-HBc and IgG subclasses (IgG1 and IgG2a) were detected by ELISA and HBcAg-specific CTL activity of mouse spleen cells were measured by 51 chromium release assay. Results The DNA vaccine could express HBcAg in vitro. The serum anti-HBc titers of mice vaccinated by gene gun or intramuscular injection were 1:328050 and 1:109350, respectively. The anti-HBc subtype of intramuscular injection was mainly IgG2a. The specific CTL killing rates of mice inoculated with the two methods were 53.1% and 52.8% respectively. Conclusion HBcAgDNA vaccine showed good cellular and humoral immunogenicity in H2b mice.