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本文报道小鼠肝浸液能强烈抑制人的淋巴细胞对植物血凝素(PHA)、刀豆素A(ConA)以及同种异型抗原所诱导的增生反应,而经60％饱和的硫酸铵盐析肝浸液组分,在相等蛋白浓度时,比原先的肝浸液有更强的抑制活性。说明选择性盐析能部分纯化肝脏免疫抑制因子。小鼠肝浸液还能抑制小鼠SRS瘤细胞在体外的生长,但抑制强度远不如对淋巴细胞反应的抑制。虽然肝浸液对同种和异种淋巴细胞增生反应有强大抑制作用,但是并未观察到对人T细胞花环形成功能的影响。小鼠脾细胞浸出液虽能显著地抑制同种淋巴细胞增生反应,但却不能明显抑制人的淋巴细胞对PHA和同种异型抗原的应答反应,也不能抑制小鼠SRS瘤细胞在体外的生长。
Here, we report that the liver extract from mice strongly inhibits the proliferation of human lymphocytes induced by phytohemagglutinin (PHA), concanavalin A (ConA), and alloantigen, whereas the 60% saturated ammonium sulfate salt Analysis of liver infusion components, at the same protein concentration, the original liver extract has a more inhibitory activity. Selective salting out shows partial purification of liver immunosuppressive factors. Liver extract of mice can inhibit the growth of mouse SRS tumor cells in vitro, but the inhibition intensity is far less than the inhibition of lymphocyte reaction. Although liver extracts strongly inhibited allogeneic lymphocyte hyperplasia, no effect on the formation of human T-cell rosette was observed. Although the spleen cell leachate could significantly inhibit allogeneic lymphocyte proliferation, it did not significantly inhibit the response of human lymphocytes to PHA and alloantigen, nor could it inhibit the growth of mouse SRS cells in vitro.