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本研究通过检测初治的成人免疫性血小板减少症(ITP)患者外周血中白介素-22(IL-22)及相关CD4+T细胞亚群的表达,探讨其在ITP发病机制中的作用。以我院住院治疗的40例新诊断的急性ITP患者及40例健康对照者为研究对象,应用ELISA法检测外周血血浆中IL-22的含量,采用流式细胞术检测Th1、Th17、Th22细胞亚群的比例,并分析其相关性。结果表明,新诊断的ITP患者血浆中IL-22的含量为(364.12±94.22)pg/ml,显著高于健康对照者(P<0.001);ITP患者外周血Th1细胞比例为(18.92±6.03)%,Th22细胞比例为(2.28±0.51)%,显著高于健康对照者(P<0.05);且ITP患者血浆IL-22水平与Th1和Th22细胞比例之间存在正相关(Th1:r=0.42,P=0.022;Th22:r=0.40,P=0.030);而ITP患者Th17细胞比例与健康对照者比较无明显差异,且与IL-22水平无相关性。结论:成人ITP患者外周血中IL-22水平明显升高,且与Th1、Th22细胞比例之间密切相关,提示IL-22与Th1和Th22细胞在ITP的发病中可能起着协同作用,而Th17细胞可能与ITP的发病无关。
This study was designed to investigate the role of interleukin-22 (IL-22) and related CD4 + T cell subsets in the pathogenesis of ITP by detecting the expression of interleukin-22 (IL-22) and related CD4 + T cells in peripheral blood of patients with newly diagnosed adult immune thrombocytopenia (ITP) Thirty cases of newly diagnosed acute ITP patients and 40 healthy controls hospitalized in our hospital were enrolled in this study. The levels of IL-22 in peripheral blood were detected by ELISA, and the levels of Th1, Th17 and Th22 cells were detected by flow cytometry The proportion of subpopulations, and analyze their relevance. The results showed that the level of IL-22 in plasma of newly diagnosed ITP patients was (364.12 ± 94.22) pg / ml, significantly higher than that of healthy controls (P <0.001). The proportion of Th1 cells in ITP patients was (18.92 ± 6.03) %, And the percentage of Th22 cells was (2.28 ± 0.51)%, which was significantly higher than that of healthy controls (P <0.05). The level of IL-22 in ITP patients was positively correlated with the ratio of Th1 and Th22 cells (Th1: , P = 0.022; Th22: r = 0.40, P = 0.030). There was no significant difference in the proportion of Th17 cells between ITP patients and healthy controls, and no correlation with IL-22 levels. Conclusion: The level of IL-22 in peripheral blood of adult patients with ITP is significantly increased, which is closely related to the ratio of Th1 and Th22 cells, suggesting that IL-22 and Th1 and Th22 cells may play synergistic roles in the pathogenesis of ITP, while Th17 Cells may not be related to the pathogenesis of ITP.