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目的研究山楂叶含药血清对高脂血清诱导的单核细胞与血管内皮细胞黏附能力及黏附分子表达的影响,并探讨其作用机制。方法采用虎红染色法检测药物血清对高脂诱导的人脐静脉内皮细胞(human umbilical vascular endothelial cells,HUVEC)和单核细胞株THP-1黏附的作用;用细胞ELISA法检测HUVEC表面细胞间黏附分子-1(intercelluar adhesion molecule-1,ICAM-1)、血管细胞黏附分子-1(vascular cellular adhesion molecule-1,VCAM-1)、P-选择素(P-selectin)的表达。结果山楂叶能抑制高脂诱导的HUVEC与THP-1的黏附,并下调HUVEC表面ICAM-1、VCAM-1、P-se-lectin的表达。结论山楂叶防治高脂血症的机制之一可能是下调血管内皮细胞黏附分子的表达,减少单核细胞与内皮细胞的黏附,从而抑制单核细胞迁移至血管内膜形成泡沫细胞而实现。
Objective To investigate the effect of hawthorn leaf-containing serum on the adhesion and adhesion molecule expression of monocytes and vascular endothelial cells induced by high-fat serum and to explore its mechanism. Methods The effect of drug serum on the adhesion of human umbilical vascular endothelial cells (HUVEC) and monocytic cell line THP-1 was detected by tiger red staining. The cell surface adhesion (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and P-selectin expression were detected by immunohistochemistry. Results Hawthorn leaves can inhibit the adhesion of HUVEC to THP-1 induced by high fat and down-regulate the expression of ICAM-1, VCAM-1 and P-se-lectin on the surface of HUVEC. Conclusion One of the mechanisms of hawthorn leaf prevention and treatment of hyperlipidemia may be to down-regulate the expression of vascular endothelial cell adhesion molecules, reduce the adhesion of monocytes and endothelial cells, and thus inhibit the migration of monocytes into the intima and form foam cells.