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水稻愈伤组织转移继代培养后的0—20天,生长速率较快,核酸和蛋白质含量急剧增加,且维持较高水平,核糖核酸酶和血红蛋白水解酶活性急剧下降,且维持较低活性。因此,愈伤组织的继代培养时间最好掌握在生长速率较快,核酸蛋白质含量较高、水解酶类活性较低的阶段,即在20天以内。核酸和蛋白质含量与其水解酶类(核糖核酸酶和血红蛋白水解酶)的比活之间存在着密切的关系,从而认为核糖核酸酶和血红蛋白水解酶分别在核酸和蛋白质含量下降中起着积极作用。根据信息大分子化合物的变化动态和愈伤组织外部形态变化提出愈伤组织转移继代培养过程可以分为三个阶段,即旺盛生长、稳定生长和衰老阶段。
After 0-60 days after subculture, the callus of rice grew rapidly, the content of nucleic acid and protein increased sharply and maintained at a high level, and the activity of RNase and hemoglobin hydrolase decreased sharply and maintained low activity. Therefore, the callus subculture time is best controlled at a faster growth rate, higher nucleic acid protein content, hydrolytic enzyme activity is lower stage, that is, within 20 days. There is a close relationship between the content of nucleic acids and proteins and the specific activities of their hydrolases (ribonucleases and hemoglobin hydrolases), suggesting that ribonucleases and hemoglobin hydrolases play a positive role in reducing the content of nucleic acids and proteins, respectively. According to the dynamic changes of information macromolecular compounds and external morphological changes of callus, callus tissue transfer subculture process can be divided into three stages, that is, vigorous growth, stable growth and senescence.