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近年来免疫病理学研究已证实细胞间粘附分子-1(ICAM-1)作为细胞粘附分子中免疫球蛋白超家族成员之一,通过与炎性细胞表面同族型配体之间相互作用形成粘附,在免疫监督、炎症反应和动脉粥样硬化等过程中起着重要的作用。因此,有关ICAM-1与肾小球系膜细胞增殖的相关研究对进一步阐明肾小球炎性疾病的发病机理及治疗的进展具有重大意义。 一、材料与方法 1.按经典方法培养、鉴定C57BL/6小鼠肾小球系膜细胞及巨噬细胞,选取第4～6代细胞用于本实验。 2.取系膜细胞,胰酶消化后,将制成的浓度为1.0×10~4/L细胞悬液加入24孔培养板内,正常对照组和
In recent years, immunopathological studies have demonstrated that intercellular adhesion molecule-1 (ICAM-1), as a member of the immunoglobulin superfamily of cell adhesion molecules, is formed by interaction with the cognate ligand on the inflammatory cell surface Adhesion plays an important role in immune surveillance, inflammatory response and atherosclerosis. Therefore, the relevant research on ICAM-1 and glomerular mesangial cell proliferation is of great significance to further elucidate the pathogenesis of glomerular inflammatory disease and the progress of treatment. First, materials and methods 1 according to the classic method of culture, identification of C57BL / 6 mouse mesangial cells and macrophages, select the 4th to 6th generation cells for this experiment. 2. Mesangial cells were taken, trypsinized, the concentration of 1.0 × 10 4 / L cell suspension was added to 24-well plates, the normal control group and