论文部分内容阅读
鸡粪中的含硫有机物在微生物作用下会释放出大量的硫化氢气体,危害畜禽的健康及污染环境。试验以减少鸡粪中硫化氢释放量为目的,从菹草内分离得到1株具有异养硫氧化作用的细菌,命名为JS3。16S rRNA序列分析表明菌株JS3与杀鲑气单胞菌的亲源关系最近。以硫代硫酸钠为唯一硫源时菌株JS3的硫氧化特征:碳源优先利用顺序为葡萄糖>柠檬酸钠>乙酸钠;氮源优先利用顺序为硝酸钠>氯化铵;菌株JS3氧化硫的最佳碳氮比为15∶1,此时77.82%的硫代硫酸根被转化为硫酸根;pH从4.05增加到10.05,菌株的氧化硫能力先增强后下降,当pH为9.05时,硫酸根的积累量最高,可达到4.33 g/L;当氯化钠添加量达到4.0%时对菌株JS3才产生明显影响,说明株菌具有一定的耐盐性;最佳硫代硫酸根初始浓度为2 810 mg/L,此时83.22%的硫代硫酸根被转化为硫酸根;250 mL三角瓶中装液量为75 mL时,菌株JS3是硫氧化能力最强。在硫化氢减释模拟试验中,与对照相比,菌株JS3可降低84.53%的硫化氢释放量。
Chicken manure in the sulfur-containing organic matter under the action of microorganisms will release a large amount of hydrogen sulfide gas, endanger the health of livestock and poultry and pollute the environment. In order to reduce the release of hydrogen sulfide in chicken manure, a strain of heterotrophic sulfur oxidation bacteria was isolated from the genus Triticum and named as JS3.16S rRNA sequence analysis. The results showed that strain JS3 was closely related to A. salmonicida The source of the recent relationship. Sulfuric acid oxidation of strain JS3 with sodium thiosulfate as sole sulfur source: the priority of carbon source was glucose> sodium citrate> sodium acetate; nitrogen source was sodium nitrate> ammonium chloride; strain JS3 was The optimal ratio of carbon to nitrogen was 15: 1, at which time, 77.82% of thiosulfate was converted to sulfate. When the pH was increased from 4.05 to 10.05, the capacity of sulfur oxide increased firstly and then decreased. At pH 9.05, The highest accumulation reached 4.33 g / L; when the addition of sodium chloride reached 4.0%, the strain JS3 had a significant effect, indicating that the strain has some salt tolerance; the optimal initial concentration of thiosulfate is 2 810 mg / L. At this time, 83.22% of thiosulfate was converted to sulfate; strain JS3 was the most sulfur-oxidizing agent in 75 mL of 250 mL flask. In the hydrogen sulfide reduction simulation test, the strain JS3 can reduce the amount of hydrogen sulfide released by 84.53% compared with the control.