为研究肝肿瘤细胞系表达FasL逃逸免疫监视的可能 ,采用逆转录 PCR方法和免疫组化法测肝癌细胞株FasL的表达 ,并测T淋巴细胞的凋亡。结果发现HEpG2细胞在mRNA水平和蛋白质水平上皆可检测到FasL的高表达 ,而 772 1细胞未能测到。HEpG2细胞与JurkatT淋巴细胞共培养后 ,19 4%的T细胞发生凋亡 ,抗FasL的单抗能阻断这种凋亡。因而肝肿瘤细胞HEpG2表达功能性的FasL ,能杀伤Fas阳性[1] 的T淋巴细胞。 In order to study the possibility of FasL escape immune surveillance in liver tumor cell lines, the expression of FasL in hepatocellular carcinoma cell lines was measured by reverse transcription PCR and immunohistochemistry, and the apoptosis of T lymphocytes was measured. The results showed that high expression of FasL was detected in both mRNA level and protein level in HEpG2 cells, whereas 7721 cells could not be detected. After co-culture of HEpG2 cells with Jurkat T lymphocytes, apoptosis occurred in 19% of T cells, and anti-FasL mAbs blocked this apoptosis. Thus, liver tumor cell HEpG2 expression functional FasL, can kill Fas positive [1] T lymphocytes.