论文部分内容阅读
目的:分析Notch信号配体——Delta样配体4(DLL4)在人脑胶质瘤中表达及其与肿瘤恶性程度、微血管密度(MVD)之间的关系,探讨DLL4在肿瘤血管生成中的作用,及DLL4作为抗肿瘤治疗靶点的可能性。方法:免疫组织化学方法检测36例人脑胶质瘤及7例正常脑组织中DLL4和血管内皮生长因子(VEGF)表达情况,用CD31标记血管内皮细胞以计算MVD。结果:DLL4及VEGF表达水平在正常脑组织、高度恶性和低度恶性胶质瘤组两两之间均有差异(P<0.01),其表达随胶质瘤恶性程度升高而增强(P<0.01);DLL4和VEGF表达之间呈正相关(r=0.539,P<0.01),两者均与MVD相关。结论:DLL4在胶质瘤的血管生成中发挥重要作用,有望成为肿瘤抗血管生成治疗的靶点。
OBJECTIVE: To analyze the expression of Notch signaling ligand, DLL4 in human glioma and its relationship with the degree of malignancy and microvessel density (MVD), and to explore the role of DLL4 in tumor angiogenesis And the potential of DLL4 as a target for anti-cancer therapy. Methods: The expression of DLL4 and vascular endothelial growth factor (VEGF) in 36 human gliomas and 7 normal brain tissues were detected by immunohistochemistry. The vascular endothelial cells were labeled with CD31 to calculate MVD. Results: The expression of DLL4 and VEGF in normal brain tissue, high grade and low grade glioma group were significantly different between two groups (P <0.01). The expression of DLL4 and VEGF increased with the malignant degree of glioma (P < 0.01). There was a positive correlation between DLL4 and VEGF (r = 0.539, P <0.01), both of which correlated with MVD. Conclusion: DLL4 plays an important role in glioma angiogenesis and is expected to be the target of anti-angiogenesis therapy.