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本研究应用细胞化学和形态计量学的实验手段，对６０Ｃｏγ射线及苯并芘在体外诱发人胚肺细胞转化过程中，细胞核内ＡｇＮＯＲｓ颗粒数量、大小等形态计量学参数的改变进行了研究，并对其生物学意义进行了分析。结果表明，γ射线和苯并芘均可在体外诱发人胚肺细胞发生转化。γ射线和苯并芘作用后早期，当人胚肺细胞尚未发生转化时，细胞核内ＡｇＮＯＲｓ颗粒计数及部分形态计量学参数已发生明显改变，并具有一定的剂量效应关系。这些规律性的变化，可用于定量地研究辐射或化学致癌因子体外诱发细胞转化的剂量效应关系，亦可望成为早期观察细胞转化程度的指标。物理化学因素复合效应研究表明，γ射线和苯并芘复合作用，对人胚肺细胞转化有复合加强作用，同时对细胞内ＡｇＮＯＲｓ颗粒数及形态计量学参数的改变亦表现出复合加强的使用。
In this study, cytochemical and morphometric methods were used to study changes in morphometric parameters such as the number and size of AgNORs in the nucleus of 60Co γ-rays and benzopyrene-induced human embryonic lung cell transformation in vitro. Its biological significance was analyzed. The results showed that both γ-rays and benzopyrene induced transformation of human embryonic lung cells in vitro. In the early stage after gamma irradiation and benzo(a)pyrene, when the human embryonic lung cells have not been transformed, the counts of AgNORs particles and some morphometric parameters have changed significantly, and have a certain dose-effect relationship. These regular changes can be used to quantitatively study the dose-response relationship of radiation-induced or chemical oncogenic factors inducing cell transformation in vitro, and may also be an indicator of early observation of cell transformation. The composite effect of physico-chemical factors showed that the combined effect of γ-rays and benzo(a)pyrene has a compounding effect on the transformation of human embryonic lung cells, and at the same time it also shows the use of compound enhancement in the change of AgNORs particle number and morphometric parameters in cells.