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胎儿出生前的诊断,主要是根据羊膜细胞培养的研究。羊膜穿刺放液术并非绝对安全,其细胞培养也要花费大量时间。此外,培养的组织细胞并不表现所有的基因。近几年来,在孕妇的血循环中发现有胎儿细胞,可能是淋巴细胞。如果能分离出这种胎儿细胞,使之浓缩,并在体外细胞培养条件下繁殖起来,那么,就能够设想用另一种方法研究胎儿疾病。如果这种方法证实是可行的,那就必须排除沾染的来源和来自母体细胞的混淆。我们所探讨的问题之一是寻求一种促细胞分裂剂,能选择性地刺激胎儿细胞,而不刺激母体细胞。我们认为,脂多糖(PLS)
Prenatal diagnosis of the fetus, mainly based on amniotic cell culture research. Amniocentesis is not absolutely safe, and its cell culture can take a long time. In addition, cultured tissue cells do not express all of the genes. In recent years, fetal cells have been found in the blood circulation of pregnant women, which may be lymphocytes. If such fetal cells can be isolated, concentrated, and propagated under in vitro cell culture conditions, then one can imagine another way to study fetal disease. If this method proves to be viable, then the source of the contamination and confusion from the maternal cells must be ruled out. One of the problems we have explored is to look for a mitogen that selectively stimulates fetal cells without stimulating maternal cells. We believe that lipopolysaccharide (PLS)