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目的 观察酸灌注对兔食管下端括约肌 (L ES)压力和平滑肌细胞内游离 Ca2 + 浓度的影响 ,及酸灌注前、后 L ES平滑肌细胞在加入乙酰胆碱后细胞内 Ca2 +浓度的不同变化。 方法 将 30只家兔随机分为实验组、对照组和正常组 ,每组各 10只。实验组 :以 1m l/ min的速度灌注 0 .1mol/ L HCl 30分钟 ,共 4天 ;对照组 :以同样的步骤方法灌注生理盐水 4天 ;正常组 :未进行灌注。测定灌注前、后 L ES压力。以 Fluo- 3作为钙指示剂 ,激光共聚焦显微镜测定酸灌注前、后细胞内游离 Ca2 +浓度的变化 ,以及其对乙酰胆碱作用的不同反应。 结果 实验组酸灌注后 L ES压力明显降低。酸灌注前、后实验组和对照组 L ES平滑肌细胞成活性无明显变化 ,其成活率均在 90 %以上。实验组细胞内游离 Ca2 +浓度明显降低 (P<0 .0 1)。在应用乙酰胆碱时正常组和对照组 L ES平滑肌细胞内游离 Ca2 +浓度明显升高(P<0 .0 1) ,而实验组则无明显变化。 结论 酸灌注降低兔 L ES压力及平滑肌细胞内游离 Ca2 + 浓度 ,影响 L ES平滑肌细胞内 Ca2 + 的贮存和释放
Objective To observe the effect of acid perfusion on the pressure of lower esophageal sphincter (L ES) in rabbits and the intracellular free Ca2 + concentration in smooth muscle cells and the changes of intracellular Ca2 + concentration in L ES smooth muscle cells before and after acid perfusion. Methods Thirty rabbits were randomly divided into experimental group, control group and normal group, with 10 in each group. Experimental group: 0.1mol / L HCl was infused at a speed of 1m l / min for 30 minutes for 4 days; control group: normal saline was infused by the same procedure for 4 days; normal group: no perfusion was performed. L ES pressure was measured before and after perfusion. The Fluo-3 was used as a calcium indicator, and the change of intracellular free Ca2 + concentration before and after acid perfusion was measured by laser confocal microscopy and its different response to acetylcholine. Results The LES pressure of experimental group decreased significantly after acid perfusion. There was no significant change in the viability of L ES smooth muscle cells in the experimental group and the control group before and after acid perfusion, with the survival rates above 90%. The concentration of intracellular free Ca2 + in the experimental group was significantly decreased (P <0.01). When using acetylcholine, the concentration of free Ca2 + in L ES smooth muscle cells in normal and control groups was significantly increased (P <0.01), while the experimental group had no significant change. Conclusion Acid infusion decreased the L ES pressure and the intracellular free Ca2 + concentration in smooth muscle cells, which affected the storage and release of Ca2 + in L ES smooth muscle cells