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目的：探讨放线共生放线杆菌表面相关物质对成纤维细胞Ｌ９２９的细胞毒性作用。方法：采用ＭＴＴ法、细胞计数法进行细胞毒性检测。结果：放线共生放线杆菌表面相关物质可明显抑制成纤维细胞Ｌ９２９的分裂、增殖，并呈明显的剂量依赖性，１００ｍｇ／Ｌ浓度组与对照组差别显著（Ｐ＜０．０５），但不致细胞死亡。结论：放线共生放线杆菌表面相关物质对Ｌ９２９细胞有明显的毒性作用，其致病机理不同于ＬＰＳ。
Objective: To investigate the cytotoxic effect of surface-associated substances of Actinobacillus actinomycetemcomitans on fibroblast L929 cells. Methods: Cytotoxicity was detected by MTT assay and cell counting method. Results: The related substances on the surface of Actinobacillus actinomycetemcomitans could significantly inhibit the proliferation and proliferation of L929 fibroblasts in a dose-dependent manner. The concentration of 100 mg / L was significantly different from that of the control group (P <0.05) Do not cause cell death. Conclusion: Actinobacillus actinomycetes surface-related substances have obvious toxic effects on L929 cells, its pathogenic mechanism is different from LPS.