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采用生物学培养性状、致病力测定和ISSR分子标记方法研究15个棉花黄萎病代表菌株的遗传变异。结果表明,供试菌株的生长速率、孢子产量与致病力的强弱呈正相关。致病力测定结果显示,致病力强的Ⅰ型有7个菌株,占46.67%,平均病情指数大于36.1;致病力弱的Ⅱ型有3个菌株,占20.00%,平均病情指数在21以下;致病力中等的Ⅲ型有5个菌株,占33.33%,平均病情指数在20～28之间。用4条ISSR引物对这些菌株进行PCR扩增,共得到623个条带,具多态性的有425条。聚类分析和相似系数分析结果显示,在0.55遗传相似水平下,供试菌株分为2个遗传类型,遗传类型与菌株致病力类型存在明显的相关性,与菌株地理来源也具有一定的相关性。
The genetic variation of 15 representative strains of Verticillium dahliae was studied using biological culture traits, pathogenicity determination and ISSR molecular markers. The results showed that the growth rate of test strains, spore yield and the strength of the disease was positively correlated. The results of pathogenicity test showed that there were 7 strains of type Ⅰ with high pathogenicity, accounting for 46.67%, the average disease index was more than 36.1; the pathogenicity of type Ⅱ was 3 strains, accounting for 20.00%; the average disease index was 21 The moderate pathogenicity of type Ⅲ 5 strains, accounting for 33.33%, the average disease index between 20 to 28. Four ISSR primers were used to amplify these strains. A total of 623 bands were obtained, of which 425 were polymorphic. Cluster analysis and similarity coefficient analysis showed that under the genetic similarity level of 0.55, the tested strains were divided into two genetic types, and the genetic types were significantly correlated with the pathogenicity of the strains, and were also related to the geographical origin of the strains Sex.