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传统的切片仅仅能够显示样品的平面结构,不能用于细胞中三维网络结构的研究.笔者在DGD diethyleneglycoldistearate 包埋-去包埋的基础上,结合电镜免疫胶体金技术对大卫百合花粉母细胞胞间及胞内细胞的骨架系统进行了研究,观察到高反差细胞微梁结构的三维网络,actin这一细胞骨架的主要成员被定位在该微梁结构纤维上.三维结构上的研究表明,actin不但是植物细胞核及细胞质骨架的成员,而且也存在于胞间连接结构 胞质桥和胞间连丝 中,推测它可能与细胞融合有关.实验结果同时表明,三维结构免疫胶体金技术对于细胞骨架和核基质的结构蛋白研究是行之有效的.
The traditional slicing can only display the planar structure of the sample and can not be used for the study of the three-dimensional network structure in the cell.Based on the embedding-embedding of DGD diethyleneglycoldistearate and the combination of electron microscopy and immunogold technique, And intracellular cytoskeletal system was studied, the high-contrast cell microbeam three-dimensional network was observed, actin the cytoskeleton main members are located in the microbeam fiber structure.Studies on the three-dimensional structure, actin Not only is it a member of the plant nucleus and cytoplasmic skeleton, but also exists in the cytoplasmic and intercellular wires of the intercellular junction structure, suggesting that it may be related to cell fusion.The experimental results also show that three-dimensional structure of immune colloidal gold technology for the cytoskeleton Studies on the structural proteins of the nuclear matrix have worked well.