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免疫过氧化物酶技术是近十多年来发展起来的一种新的免疫组织化学技术,由于它比免疫荧光和免疫铁蛋白技术有较多的优点,近几年来发展很快,已广泛应用于细胞生物学、病理学和医学理论方面的研究。一、几种免疫过氧化物酶的方法目前免疫过氧化物酶的技术已有多种(图1)。每一种都是利用特异的抗原抗体反应和辣根过氧化物酶(HRP)的活牲。HRP在H_2O_2存在的条件下,分解H_2O_2而使某些化合物(底物)氧化成有色的不溶性产物,沉着在抗原的部位。因此应用这种技术能在显微镜下观察组织和细胞内抗原的分布和定位。
Immunoperoxidase technology is a new immunohistochemical technique developed in recent ten years. Because of its advantages over immunofluorescence and immunoferritin technology, it has developed rapidly in recent years and has been widely used Research in cell biology, pathology and medical theory. First, several methods of immune peroxidase Currently peroxidase immune technology has a variety of (Figure 1). Each uses specific antigen-antibody reactions and horseradish peroxidase (HRP) activity. In the presence of H 2 O 2, HRP decomposes H 2 O 2 to oxidize some of the compounds (substrates) into colored insoluble products and settle at the antigenic site. Therefore, the use of this technology can be observed under the microscope tissue and intracellular antigen distribution and positioning.