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背景:国内外有关内皮祖细胞的分离培养和鉴定方面的文章很多,但是人脐血和外周血内皮祖细胞的鉴别方面文章不多。目的:从人脐血和外周血中分离出内皮祖细胞,并对其进行培养和鉴定。方法:选取密度梯度离心法从脐血和外周血中分离获得单个核细胞,按照1×106/cm2的浓度种植于预先铺有纤维连接蛋白的培养板中,用含血管内皮生长因子的M199培养基进行诱导培养。结果与结论:人脐血和外周血中存在内皮祖细胞,浓度梯度离心联合贴壁筛选获得的单个核细胞在血管内皮生长因子的诱导培养下可分化成内皮祖细胞,内皮祖细胞表达CD34、CD133、CD105、KDR和CD31,能吞噬乙酰化低密度脂蛋白,结合荆豆凝集素1,它们可作为体外分选内皮祖细胞的标志。
BACKGROUND: There are many articles on the isolation, culture and identification of endothelial progenitor cells both at home and abroad, but there are not many articles on the identification of human umbilical cord blood and peripheral blood progenitor cells. Objective: To isolate endothelial progenitor cells from human umbilical cord blood and peripheral blood and culture and identify them. Methods: Mononuclear cells were isolated from cord blood and peripheral blood by density gradient centrifugation. The cells were seeded at a density of 1 × 106 / cm2 in fibronectin-plated plates and cultured with vascular endothelial growth factor M199 Induction culture. RESULTS AND CONCLUSION: The endothelial progenitor cells were present in human umbilical cord blood and peripheral blood. Mononuclear cells obtained by concentration gradient centrifugation and adherent screening could differentiate into endothelial progenitor cells under the induction of vascular endothelial growth factor. The endothelial progenitor cells expressed CD34, CD133, CD105, KDR and CD31, which are capable of phagocytosing acetylated LDL and binding to Vitexin 1, can be used as markers for in vitro sorting of endothelial progenitor cells.