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黄嘌呤-黄嘌呤氧化酶系统(X-XOD)可诱发培养心肌细胞的自由基损伤。鉴于钼是黄嘌呤氧化酶(XOD)的重要组分,而且钼对于心肌细胞有保护作用还是有损伤作用的报道不尽一致,本实验拟以心肌细胞动作电位与自发性搏动为指标,探讨钼对心肌细胞机能影响的规律。 实验方法取新生昆明种小鼠与Wistar大鼠的乳鼠;胎龄4～6个月,水囊引产的人胚心尖,分别经0.1％胰蛋白酶分离心肌细胞,然后将其置入聚苯乙烯培养瓶,在36.5℃、5％CO_2和95％空气的孵箱内培养。常规培养基由80％199及20％小牛血清组成。小鼠心肌细
Xanthine-xanthine oxidase system (X-XOD) induces free radical damage in cultured cardiomyocytes. In view of molybdenum is an important component of xanthine oxidase (XOD), and molybdenum have protective effect on cardiomyocytes are still not the same as the role of injury reported in this experiment to cardiomyocyte action potential and spontaneous pulsatility as an indicator to explore the molybdenum Cardiac function of the law. Experimental methods Take neonatal Kunming mice and Wistar rats in neonatal rats; gestational age 4 to 6 months, the epididymal heart apex induced by 0.1% trypsin, respectively, and then placed in polystyrene Flasks were cultured in an incubator at 36.5 ° C, 5% CO 2 and 95% air. Normal medium consists of 80% 199 and 20% calf serum. Small mouse heart muscle