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采用ＰＣＲ、ＰＣＲ－ＲＦＬＰ等方法检测５１例宫颈组织（其中宫颈癌标本３８例，正常宫颈组织标本１３例）中高危型人乳头瘤病毒（ＨＰＶ）－１６，１８感染情况及Ｈ－ｒａｓ基因突变情况。研究结果显示，宫颈组织中ＨＰＶ－１６，１８感染率为７４％（２８／３８），表明高危型ＨＰＶ－１６，１８是宫颈癌发生的重要因素。Ｈ－ｒａｓ基因突变率为２９％（１１／３８）。正常宫颈组织中上述检测结果均为阴性。Ｈ－ｒａｓ基因突变与多种肿瘤发生有关；显示该基因突变与宫颈癌发生有关，但不是早期事件，也不能作为临床恶变与预后判断的指标；Ｈ－ｒａｓ基因突变与ＨＰＶ－１６，１８型感染具有相关性
PCR and PCR-RFLP were used to detect high-risk human papillomavirus (HPV)-16,18 infection and H-ras gene mutations in 51 cases of cervical tissue (including 38 cervical cancer specimens and 13 normal cervical specimens). Happening. The results showed that the infection rate of HPV-16, 18 in cervical tissue was 74% (28/38), indicating that high-risk HPV-16, 18 is an important factor in the occurrence of cervical cancer. The H-ras gene mutation rate was 29% (11/38). The above test results were negative in normal cervical tissue. H-ras gene mutations are associated with multiple tumorigenesis; this gene mutation is associated with cervical cancer, but it is not an early event and cannot be used as an indicator of clinical malignancy and prognosis. H-ras gene mutation is associated with HPV-16, type 18 Infection is related