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目的:探讨阿托伐他汀对冠心病大鼠心肌细胞的凋亡及caspase-12表达的影响。方法:21只清洁剂SD大鼠经高脂饮食冠心病造模成功后随机分为空白组、对照组和阿托伐他汀组每组7只。空白组大鼠未经处理,对照组给予生理盐水10 m L/kg体重灌胃,1天一次连续4周;阿托伐他汀组大鼠给予阿托伐他汀10 mg/kg体重灌胃,1天一次连续4周。4周后处死大鼠并取大鼠心肌组织采用TUNEL法检测细胞凋亡情况,Western-blot法检测caspase-12表达情况。结果:三组大鼠心肌凋亡比较空白组、对照组及阿托伐他汀组大鼠细胞凋亡比例分别为(3.72±0.89)%、(3.84±1.01)%和(1.47±0.62)%,阿托伐他汀大鼠心肌细胞凋亡比例明显低于空白组和对照组(P<0.05);阿托伐他汀组大鼠心肌细胞Caspase-12表达水平显著低于空白组与对照组(P<0.05),而空白组与对照组比较无统计学差异(P>0.05)。结论:阿托伐他汀可能通过抑制大鼠心肌细胞caspase-12表达来阻断心肌细胞凋亡途径的激活。
Objective: To investigate the effect of atorvastatin on cardiomyocyte apoptosis and caspase-12 expression in rats with coronary heart disease. Methods: Twenty-one SD rats were randomly divided into blank group, control group and atorvastatin group. The rats in the blank group were untreated, the rats in the control group were given normal saline at a dose of 10 m L / kg body weight once a day for 4 weeks, the rats in the atorvastatin group were given orally atorvastatin 10 mg / kg body weight, Day for 4 consecutive weeks. After 4 weeks, the rats were sacrificed and the myocardial tissue was taken for detecting apoptosis by TUNEL method. The expression of caspase-12 was detected by Western-blot. Results: Compared with blank control group, the apoptotic rates in the three groups were (3.72 ± 0.89)%, (3.84 ± 1.01)% and (1.47 ± 0.62)% respectively in the control group and atorvastatin group, The percentage of apoptotic cardiomyocytes in atorvastatin group was significantly lower than that in blank group and control group (P <0.05). Caspase-12 expression in atorvastatin group was significantly lower than that in blank group and control group (P < 0.05), while there was no significant difference between the blank group and the control group (P> 0.05). Conclusion: Atorvastatin may block the activation of apoptotic pathway in cardiomyocytes by inhibiting caspase-12 expression in rat cardiomyocytes.