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通过建立荧光猝灭法来检测皮革中痕量Cr(VI)的方法,为皮革中Cr(VI)的检测提供另一种选择。首先通过分析得到检测的最佳条件:测试温度为20℃;缓冲溶液pH为7.0,用量为2 mL;荧光素用量为1.0 mL;KI用量为2.0 mL;KI与Cr(VI)反应时间为15 min;静置时间为15 min。同时得到了测定Cr(VI)的回归方程为△F=6.0571C(μg/L)-5.2,线性相关系数r=0.9939,线性范围为4~60μg/L,检出限为8.4μg/L,精密度为2.01%,加标回收率大于91%。利用所建立的荧光猝灭法分析了6个不同Cr(VI)含量的皮革样品,并与紫外分光光度法对比,相对误差小于5%,说明该方法适用于皮革中Cr(VI)含量的测定。
Through the establishment of fluorescence quenching method to detect trace amounts of Cr (VI) in leather, it provides another choice for the detection of Cr (VI) in leather. First of all, the best detection conditions were as follows: the temperature was 20 ℃, the buffer pH was 7.0, the dosage was 2 mL, the amount of fluorescein was 1.0 mL, the KI was 2.0 mL, and the reaction time of KI and Cr (VI) was 15 min; standing time is 15 min. At the same time, the regression equation of Cr (VI) was found to be △ F = 6.0571C (μg / L) -5.2, the linear correlation coefficient was 0.9939, the linear range was 4 ~ 60μg / L and the detection limit was 8.4μg / The precision was 2.01%, the spiked recovery was more than 91%. Six samples of leather with different contents of Cr (VI) were analyzed by fluorescence quenching method. The relative error was less than 5% compared with UV spectrophotometry, indicating that the method is suitable for the determination of Cr (VI) in leather .