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利用发根农杆菌 R16 0 1侵染龙眼 ,研究不同基因型及不同外植体对转化的影响 .结果表明 :R16 0 1对龙眼红核子和乌龙岭 2个品种都有较强的侵染力 ,侵染率高且重复性好 ,在无菌苗及胚状体上都诱导出大量的毛状根 ;成熟 1月龄的胚状体是转化的良好受体 ;切割处理及低盐培养基有利于毛状根的诱导和生长
The effects of different genotypes and different explants on the transformation were studied by Agrobacterium rhizogenes R16 0 1 infection.The results showed that R16 0 1 had strong infection on both longan and Wulongling High infectivity and good repeatability. A large number of hairy roots were induced in both sterile seedlings and embryoid bodies. One-month mature embryoid bodies were good recipients for transformation. Cutting and low salt culture Base is conducive to hairy root induction and growth