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目的分析Eppin抗原的二级结构并预测其B细胞表位。方法联合运用多种方法对Eppin的二级结构和表面特性,如亲水性、理化性质、可及性、免疫原性和可塑性等方面进行分析,预测其抗原表位。结果Eppin存在多个潜在的抗原表位位点,可能的蛋白质抗原表位区域:14～23,30～44,48～54,56～68,78～85,97～106,109～116,125～132。体外实验证明,所预测抗原表位区域基本能与免疫抗血清发生抗原特异性反应。结论应用多参数成功预测了Eppin抗原的B细胞表位。
Objective To analyze the secondary structure of Eppin antigen and predict its B cell epitope. Methods The secondary structure and surface properties of Eppin, such as hydrophilicity, physico-chemical properties, accessibility, immunogenicity and plasticity, were analyzed in combination with various methods to predict the epitope of Eppin. Results There were several potential epitopes for Eppin. The possible protein epitopes were 14-23, 30-44, 48-54, 56-68, 78-85, 97-106, 109-116, 125-132. In vitro experiments show that the predicted antigen epitopes can react with immune antisera antigen-specific reaction. Conclusion The B cell epitopes of Eppin antigen were successfully predicted by using multiple parameters.