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温补培元方是由人参、黄芪、附子、黄柏、山萸肉等13味中药组成的。是从新安温补培元医家汪机、孙一奎、郑重光、吴楚等8位医家653例运用温补培元法的医案中,用Foxpro编译程序进行统计,并根据这些医家的配伍规律,总结而成的。为了进一步研究该方温补培元的作用机理,我们用利血平复翩成小鼠脾虚模型,用该方作用后,观察对脾虚型小鼠机体巨噬细胞吞噬作用、脾脏细胞NK活性和conA刺激的淋巴细胞掺入率影响,以阐明该方对改善脾虚证的意义。 材料 1、动物实验动物用近交系C_(57)BL小鼠,6~8周龄,体重22±4g左右,雌雄兼用,由中国科学院上海实验动物中心提供。实验前在本校实验中心饲养1
Wenbu Peiyuan is composed of 13 Chinese herbs, including ginseng, astragalus, aconite, cork, and hawthorn meat. The statistics were compiled by Foxpro compilers from 653 medical practitioners, including Wang Ji, Sun Yikui, Zheng Zhongguang, and Wu Chu, in Xinanwen, using the Wen Bu Pei Yuan method. According to the compatibility rules of these physicians, Summarized. In order to further study the mechanism of this side Wenpaiyuan, we used the model of spleen deficiency of mice reconstituted into reserpine and used this side to observe the macrophage phagocytosis, spleen cell NK activity and conA on spleen deficiency mice. The effect of stimulated lymphocyte incorporation was clarified to clarify the significance of this prescription for improving spleen deficiency syndrome. Materials 1. Inbred C_(57)BL mice of animal experimental animals, 6-8 weeks old, weighing 22±4g, both male and female, provided by Shanghai Experimental Animal Center of Chinese Academy of Sciences. Pre-experimentation at the school’s experimental center 1