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目的:建立HPLC法测定四叶参中四叶参皂苷的含量测定方法。方法:色谱柱为Kromasil C_(18)(250 mm×4.6 mm,5μm);流动相为0.1%磷酸水-乙腈(70∶30);流速为1.0 ml·min~(-1);柱温为30℃;检测波长为203 nm。结果:四叶参中四叶参皂苷与其他组分分离良好,线性范围为1.09~17.41μg(r=0.999 9),加样回收率为98.46%(RSD=1.64%,n=6)。结论:该方法准确性、重复性好,可用于四叶参中四叶参皂苷的含量测定。
Objective: To establish a HPLC method for the determination of four leaf ginseng ginsenoside determination. Methods: The chromatographic column was Kromasil C 18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.1% phosphoric acid water (70:30) and the flow rate was 1.0 ml · min -1 .The column temperature was 30 ℃; detection wavelength of 203 nm. Results: The four-leaf ginseng saponins isolated from other components with good linear range of 1.09 ~ 17.41μg (r = 0.999 9), sample recovery was 98.46% (RSD = 1.64%, n = 6). Conclusion: The method is accurate, reproducible and can be used for the determination of four leaf ginseng ginseng saponins content.