论文部分内容阅读
鉴于有性世代形成能高的稻瘟病菌菌株有限,稻瘟病菌间的杂交组合难于获得,从而很难对一些重要基因,如无毒基因,抗药性基因等进行遗传分析及深入研究。本研究利用原生质体融合技术获得梨孢菌的杂合子,从而评价该体系用于遗传研究的可行性。首先,利用转基因技术获得稻瘟病菌Y93-164a-1跟蟋蟀草病菌SA98-4的抗药性转化子,然后将两个菌的稳定转化子的原生质体进行融合,之后进行连续5次的继代培养,最后进行3次单孢分离来挑选稳定的融合子。结果表明,利用原生质体融合技术在两个不同致病型的梨孢菌之间能获得稳定的融合子,初步表明利用原生质体融合技术组建梨孢菌的融合子群体用于遗传分析在技术上是可行的。
In view of the limited number of M. grisea strains capable of forming sexual generation, it is difficult to obtain hybrid combinations between M. grisea and it is difficult to carry out genetic analysis and further study of some important genes, such as non-toxic genes and resistance genes. In this study, we used the protoplast fusion technology to obtain the heterozygote of the pear spores, and then evaluated the feasibility of the system for genetic research. First, the resistant transformants of Magnaporthe grisea Y93-164a-1 and S. cricket strain SA98-4 were obtained by using the transgenic technique, then the protoplasts of the stable transformants of the two strains were fused, followed by five consecutive passages Finally, three single-spore isolations were performed to select stable fusions. The results showed that the use of protoplast fusion technology in two different pathogenic types of pear spores can obtain stable fusion, preliminary shows that the use of protoplast fusion technology to establish a population of fusion spores of the pear for genetic analysis in the technical It works.