选择毛细管区带电泳分离模式 ,以肉桂酸为内标建立了适合板蓝根药材中抗内毒素活性成分五种有机酸优化分离与定量分析的毛细管电泳方法。电泳分离条件 :毛细管 4 0 cm× 50 μm,运行缓冲液 p H9.5磷酸二氢钠 -硼砂 ( 50 mmol/L∶ 1 2 .5mmol/L,内含 1 6mmol/Lβ-CD) ,压力进样 2 0 psi× s,操作电压 1 8k V ( +)→( -) ,柱上在线检测 UV2 0 0 nm,毛细管柱温 2 5℃ The capillary zone electrophoresis separation mode was selected and the cinnamic acid was used as the internal standard. A capillary electrophoresis method was developed to optimize the separation and quantification of five organic acids with anti-endotoxin activity components in Radix Isatidis. Electrophoretic separation conditions: capillary 40 cm × 50 μm, running buffer p H9.5 sodium dihydrogen phosphate-borax (50 mmol/L: 12.5 mmol/L, containing 16 mmol/L β-CD), pressure Sample 20 psi× s, operating voltage 1 8k V (+)→(-), on-line detection UV2 0 nm, capillary column temperature 25°C