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目的:建立定风止痛片中三七皂苷R1、人参皂苷Rg1、Rb1的含量测定方法。方法:采用Hyper-sil ODS C18色谱柱(150mm×4.6mm,5μm);流动相为乙腈-水,梯度洗脱;检测波长203nm,流速1.0mL.min-1,柱温20℃。结果:三七皂苷R1在1.02～10.15μg/mL范围内呈良好的线性关系,回归方程为:Y=4932.4X+21.78(r=0.9996),加样回收率为99.91%(RSD=1.18%,n=6);人参皂苷Rg1在3.82～38.24μg/mL范围内呈良好的线性关系,回归方程为:Y=1415.9X+9.496(r=0.9998),加样回收率为100.08%(RSD=0.68%,n=6);人参皂苷Rb1在3.04～30.36μg/mL范围内呈良好的线性关系,回归方程为:Y=3118.7X+2.913(r=0.9994),加样回收率为100.30%(RSD=1.45%,n=6)。结论:该法操作简便,结果准确,可以用于测定定风止痛片中的三七皂苷R1、人参皂苷Rg1、Rb1含量,为定风止痛片的质量控制提供参考。
Objective: To establish a method for the determination of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Dingfeng Zhitong Tablet. METHODS: Hyper-sil ODS C18 column (150 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-water with gradient elution. The detection wavelength was 203 nm and the flow rate was 1.0 mL · min-1. Results: The content of notoginsenoside R1 showed a good linearity in the range of 1.02-10.15 μg / mL. The regression equation was Y = 4932.4X + 21.78 (r = 0.9996), the recoveries were 99.91% (RSD = 1.18% n = 6). Ginsenoside Rg1 showed a good linearity in the range of 3.82-38.24μg / mL. The regression equation was Y = 1415.9X + 9.496 (r = 0.9998), the recovery was 100.08% %, n = 6). The linear range of ginsenoside Rb1 was 3.04 ~ 30.36μg / mL. The regression equation was Y = 3118.7X + 2.913 (r = 0.9994) = 1.45%, n = 6). Conclusion: The method is simple and accurate. It can be used to determine the content of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Dingfeng Zhitong Tablet and provide reference for the quality control of Dingfeng Zhitong Tablet.