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慢活肝患者的血清,以自己或非自己的淋巴细胞作靶细胞进行细胞毒性试验,结果患者血清对淋巴细胞有较高的毒性。而同一试验慢性非活动性肝炎和正常人的血清对淋巴细胞几乎没有细胞毒性。提示慢活肝患者血清中存在淋巴细胞毒性因子,这个毒性因子是一种免疫球蛋白M。本试验对胸腺细胞毒性更高,在系统性红斑狼疮患者,孕妇和法国豚鼠血清中证实这个因子和T细胞毒性抗体有相同的性质。由于慢活肝患者血清中存在这个因子,故在同种疾病患者,之所以未梢血T细胞减少,可能和细胞免疫机能低下有关。使用肝活检确诊慢活肝、慢性非活动性肝炎、肝硬变、肝癌和其它肝脏疾病者以及正常人。作者按B(?)yum A,1968年的方法分离淋巴细胞,要求淋巴细胞悬液5×10~6细胞/ml,根据Terasaki 1970年的原理和方法,被检血清要灭活,分别以自己或他人的
Serum of slow-acting liver patients, with their own or non-own lymphocytes as target cells for cytotoxicity test, the results of patients with high serum lymphocyte toxicity. Serum from patients with chronic non-active hepatitis and normal people in the same experiment showed almost no cytotoxicity to lymphocytes. It is suggested that there is lymphocyte cytotoxicity in the serum of patients with chronic liver disease, and this toxic factor is an immunoglobulin M. This test is more toxic to thymocytes, confirming that this factor shares the same properties as T-cell cytotoxic antibodies in patients with systemic lupus erythematosus, pregnant women and French guinea pig serum. Due to the presence of this factor in the serum of slow-acting liver patients, the reason why there is a decrease in peripheral blood T-cells in patients with the same type of disease may be related to the poor cellular immunity. Use of liver biopsy diagnosed with slow-living liver, chronic non-active hepatitis, cirrhosis, liver cancer and other liver diseases and normal people. According to the method of B (?) Yum A, lymphocytes were isolated by the method of B (?) Yum A in 1968 and required 5 × 10 ~ 6 cells / ml of lymphocyte suspension. According to the principle and method of Terasaki 1970, the sera were inactivated, Or others