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心肌梗塞的诊断一般依靠病史、心电图和血清酶的检查,但每一项的敏感性和特异性仍有缺陷。Gere等报道在心肌梗塞患者的血清中,心肌肌凝蛋白轻链的数量增多。本文报道应用放射免疫方法检测血清心肌肌凝蛋白轻链以诊断急性心肌梗塞的结果。方法:118例主诉胸痛而收入冠心监护病室的患者,根据其心电图改变分为无心肌梗塞、急性心肌梗塞和未分类三组,测定其血清中的肌酸激酶和心肌肌凝蛋白轻链;12名正常对照者亦作了测定。心肌肌凝蛋白轻链是从死亡12小时以内的人体正常心肌按Wikman—Coffelt方法提取,再在SephedexA—50离子交换层析柱上纯化,抗体则通过免疫家兔
The diagnosis of myocardial infarction generally rely on history, ECG and serum enzyme tests, but each of the sensitivity and specificity are still flawed. Gere et al. Reported an increase in the number of cardiac myosin light chains in sera of patients with myocardial infarction. This article reports the use of radioimmunoassay detection of serum cardiac myosin light chain in the diagnosis of acute myocardial infarction results. Methods: One hundred and eighteen patients who complained of chest pain and were admitted to coronary care unit were divided into three groups according to their changes of electrocardiogram: no myocardial infarction, acute myocardial infarction and no classification. Their serum creatine kinase and cardiac myosin light chain were measured. Twelve normal controls were also tested. Myocardial myosin light chain was extracted from human normal myocardium within 12 hours after the death by the Wikman-Coffelt method and then purified on a Sephedex A-50 ion exchange chromatography column. The antibody was purified by immunizing rabbits