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在不含缬氨酸（ Ｌ Ｖａｌ）的 Ｍ Ｅ Ｍ 培养基基础上，以普通 Ｍ Ｅ Ｍ 培养基 Ｌ Ｖａｌ（４６ｍ ｇ／ Ｌ）和亮氨酸（ Ｌ Ｌｅｕ）含量（５２ｍ ｇ／ Ｌ）为准，配制 １２ 种限制 Ｌ Ｖａｌ、增加 Ｌ Ｌｅｕ 的不平衡支链氨基酸培养基，用 Ｍ Ｔ Ｔ法观察其对胃癌细胞株 Ｓ Ｇ Ｃ７９０１ 体外增殖的影响和对该细胞株化疗药物敏感性的影响。结果表明，限制 Ｌ Ｖａｌ至正常的 １／４ 对 Ｓ Ｇ Ｃ７９０１ 细胞即有明显的抑制作用（ Ｐ＜ ０．０１），随着 Ｌ Ｖａｌ浓度的继续降低，抑制作用更加显著。增加 Ｌ Ｌｅｕ 至正常的４ 倍，显著增强了 Ｌ Ｖａｌ缺乏对 Ｓ Ｇ Ｃ７９０１ 细胞的抑制作用。不平衡支链氨基酸与抗癌药物５ Ｆ Ｕ、 Ｍ Ｍ Ｃ 和 Ｍ Ｔ Ｘ 有协同作用。提示在氨基酸疗法中，不仅要调整某种氨基酸的绝对量，而且应调整该氨基酸与其他氨基酸的比例。
Based on the M-M medium without L-Val, L-Val (46m g/L) and Leu (L-Leu) content (52m g/L) were used as normal M E M medium. L) Precisely, 12 kinds of unbalanced branched-chain amino acid media that constrain L-Val and increase L-Leu were prepared, and the effect of cell division on proliferation of gastric cancer cell line S G C-7901 in vitro was observed by M T T method and the cell The effect of chemotherapeutic drug sensitivity. The results showed that limiting 1/4 of L-Val to normal had a significant inhibitory effect on SG C-7901 cells (P<0.01), and the inhibitory effect was even more pronounced as L-Val concentration continued to decrease. Increasing L-Leu to normal 4 times significantly increased the inhibitory effect of L-Val deficiency on S G C-7901 cells. Unbalanced branched-chain amino acids and 5-FU, M M C and M T X have synergistic effects. In amino acid therapy, it is not only necessary to adjust the absolute amount of an amino acid but also to adjust the ratio of that amino acid to other amino acids.