论文部分内容阅读
目的研究自身免疫性疾病系统性红斑狼疮(SLE)中白细胞介素(IL)-23和IL-12的调节作用。方法78例SLE患者[以SLE疾病活动评分(SLEDAI)进行活动性评分]和正常对照36名,分别抽提SLE患者和正常对照外周血单个核细胞(PBMC)的mRNA,并反转录成cDNA,用实时定量聚合酶链反应(PCR)的方法对IL-23特异的亚单位P19、IL-12特异的亚单位P35以及它们共有的亚单位P40作定量分析,数据采用看家基因进行标化。结果未经治疗、已经治疗的SLE患者的IL-12特异性的亚单位P35表达水平显著低于正常对照(P值分别<0.05,<0.01)。与未经治疗相比,用类固醇激素或类固醇类激素加免疫抑制剂治疗的SLE患者显著抑制P40和P19的表达(P值分别<0.01和<0.05)。活动性SLE患者(SLEDAI>10)的P19、P40、P35表达水平显著高于非活动性SLE患者(SLEDAI≤10)(P值分别<0.01,<0.01和<0.05)。结论IL-12的表达下降或IL-23的表达上调也许参与SLE的发病过程,这两种细胞因子有可能是SLE患者的治疗靶点。
Objective To investigate the regulatory role of interleukin (IL) -23 and IL-12 in autoimmune diseases systemic lupus erythematosus (SLE). Methods Totally 78 SLE patients with SLE activity scores (SLEDAI score) and 36 normal controls were enrolled and their mRNAs were extracted from peripheral blood mononuclear cells (PBMCs) from patients with SLE and normal control and reverse transcribed into cDNA , Quantitative analysis of IL-23-specific subunit P19, IL-12-specific subunit P35 and their common subunit P40 by real-time quantitative polymerase chain reaction (PCR) was performed and the data were normalized with housekeeping genes . Results The untreated SLE patients had a significantly lower level of IL-12-specific subunit P35 expression than the normal controls (P <0.05, <0.01, respectively). SLE patients treated with steroid hormones or steroid hormones plus immunosuppressive agents significantly inhibited the expression of P40 and P19 (P <0.01 and <0.05, respectively), compared with no treatment. The levels of P19, P40 and P35 in active SLE patients (SLEDAI> 10) were significantly higher than those in inactive SLE patients (SLEDAI≤10) (P <0.01, <0.01 and <0.05, respectively). Conclusion The decreased expression of IL-12 or the upregulation of IL-23 may be involved in the pathogenesis of SLE. These two cytokines may be therapeutic targets for SLE patients.