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由文献表明:迄今为止,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定明胶分子量分布,只能分出<α,α_1,α_2和β组份,而γ及>γ的组份未能检出。也就是说,大约30万以上的分子量组份未能进入凝胶板内(以下简称“入板”)。这对明胶构象的全分析和对明胶质量的正确判断是一个严重的缺陷。 本文主要探讨和研究γ及>γ组份的入板问题。所采取的方法是:控制丙烯酰胺和甲叉丙烯酰胺(交联剂)的相对含量,选择合适的交联度梯度,使之既适用于α和β组份迁移,也能让γ及>γ的组份入板。此外,在小的交联度和大孔径的情况下,还能符合凝胶板对凝胶强度的基本要求。
From the literature it has been shown that, until now, the molecular weight distribution of gelatin has been determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with only <α, α_1, α_2 and β components being separated out and γ and> γ component failed to detect. That is, about 300,000 or more molecular weight components fail to enter the gel plate (hereinafter referred to as “plate”). This is a serious defect in the complete analysis of the conformation of gelatin and the correct judgment of the quality of the gelatin. This paper mainly discusses and studies the problem of γ and γ component into the board. The method adopted is to control the relative content of acrylamide and methylacrylamide (cross-linking agent), select appropriate gradient of the degree of cross-linking so as to be suitable for the migration of α and β components, and also to make γ and γ The ingredients into the board. In addition, with a small degree of crosslinking and a large pore size, the gel sheet can meet the basic requirements of gel strength.