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目的 :制备用于肿瘤热化疗和逆转多药耐药的As2O3磁性Fe3O4白蛋白微球并表征。方法:化学共沉淀法制备Fe3O4磁性纳米粒,运用透射电镜、X射线衍射分析进行表征,溶血实验及MTT试验进行毒理学评定。去溶剂化交联法制备As2O3磁性Fe3O4白蛋白微球,运用透射电镜和能谱仪进行表征,在交变磁场作用下进行体外升温试验,体外释药方法研究其释药速率。结果:Fe3O4磁性纳米粒近似球形,粒径约20 nm,无溶血作用,细胞毒性为1级。As2O3磁性Fe3O4白蛋白微球近似球形,大小均匀,粒径约193.4 nm,其不同浓度的磁流体在交变磁场下可升温至39.5~58.0℃并保持恒定。体外释药实验证实As2O3磁性Fe3O4白蛋白微球具有明显的缓释功能。结论:Fe3O4磁性纳米粒作为药物载体具有良好的生物相容性。用去溶剂化交联法可以成功制备出As2O3磁性Fe3O4白蛋白微球,As2O3磁性Fe3O4白蛋白微球释药速率缓慢,为研究肿瘤热化疗和逆转多药耐药提供理论和实验基础。
OBJECTIVE: To prepare and characterize As2O3 magnetic Fe3O4 albumin microspheres for hyperthermia and reversal of multidrug resistance. METHODS: Fe3O4 magnetic nanoparticles were prepared by chemical coprecipitation. The morphology of the nanoparticles was characterized by transmission electron microscopy and X-ray diffraction. The hemolysis and MTT were used to evaluate the toxicology. As2O3 magnetic Fe3O4 albumin microspheres were prepared by solvating and crosslinking. The microspheres were characterized by transmission electron microscopy and energy dispersive spectroscopy. The in vitro temperature-rising test was carried out under the effect of alternating magnetic field. The drug release rate was studied in vitro. Results: The Fe3O4 magnetic nanoparticles were approximately spherical in shape and had a particle size of about 20 nm with no hemolysis and a cytotoxicity of grade 1. As2O3 magnetic Fe3O4 albumin microspheres are nearly spherical with a uniform size of about 193.4 nm. The magnetic fluids of different concentrations can be heated to 39.5-58.0 ℃ under alternating magnetic field and remain constant. In vitro release experiments confirmed that As2O3 magnetic Fe3O4 albumin microspheres have a significant sustained-release function. Conclusion: Fe3O4 magnetic nanoparticles as a drug carrier has good biocompatibility. As2O3 magnetic Fe3O4 albumin microspheres can be successfully prepared by desolvation cross-linking method. The release rate of As2O3 magnetic Fe3O4 albumin microspheres is slow, which provides theoretical and experimental basis for the study of thermochemotherapy and reversal of multidrug resistance.