用细胞遗传学方法检查了6个来源于4例原发卵巢癌的细胞系。这些细胞系都用RPMI1640培养基和营养素维持。用标准细胞遗传学技术进行气干法制片,但秋水仙素作用时间在20分钟至30分钟之间,低渗液为1%的柠檬酸盐。制片后于37℃放置两周再进行GTG和C显带。通过染色体计数发现6个细胞系均为非整倍体,但其中1个细胞系含有两个群体,分别具有假二倍体和假四倍 Six cell lines derived from four primary ovarian cancers were examined by cytogenetics. These cell lines are maintained with RPMI 1640 medium and nutrients. Air-dried tablets were prepared using standard cytogenetic techniques but with colchicine acting between 20 and 30 minutes and hypotonic 1% citrate. After preparation for two weeks at 37 ℃ for GTG and C banding. Six cell lines were found to be aneuploid by chromosome counts, but one of the two cell lines contained two populations with pseudoforms and fake quadruple