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目的 :观察参杞合剂 (SQ)在体内、外对小鼠腹水型肝癌细胞株 (HcaF16A3 )细胞周期及凋亡的影响。方法 :用SQ对荷瘤小鼠连续胃饲治疗 10d ,观察其NK细胞 /Mφ的杀伤活性及细胞周期的改变。用流式细胞仪检测SQ对HcaF16A3 细胞增殖的抑制作用与诱导凋亡的作用。结果 :SQ的抑瘤率为6 5 .6 8%。流式细胞仪检测发现 ,SQ可使肿瘤细胞的细胞周期阻滞到S期 ,并在体外诱导其凋亡。结论 :SQ在体内、外均有抑瘤作用 ,其机制与细胞周期阻滞继而诱发凋亡有关
Objective: To observe the effect of Shenqi Mixture (SQ) on cell cycle and apoptosis of mouse ascitic hepatocellular carcinoma cell line (HcaF16A3) in vitro and in vivo. METHODS: The tumor-bearing mice were treated with SQ for 10 days. The killing activity and cell cycle of NK cells/Mφ were observed. The effect of SQ on the proliferation of HcaF16A3 cells and the induction of apoptosis were detected by flow cytometry. Results : The inhibition rate of SQ was 65.8%. Flow cytometry revealed that SQ can arrest the cell cycle of tumor cells to S phase and induce apoptosis in vitro. Conclusion: SQ has anti-tumor effect both in vitro and in vivo. Its mechanism is related to cell cycle arrest and subsequent apoptosis induction.