汶川地震对岷江柏林土壤微生物群落及养分的影响

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为了探索地震灾害对森林土壤肥力和微生物多样性的影响,以四川理县地震灾区不同受灾程度的岷江柏林土壤养分及细菌、古菌群落为调查对象,在受灾区选择2个典型土壤类型、7个人工岷江柏(Cupressus chenginana)林为调查对象,其中,在熊尔山调查点(山地褐土)选择1个受地震影响小的林分作为对照,3个受灾林分,在蒲溪沟调查点(山地棕壤)选择1个对照和2个受灾林分。结果表明:受地震影响,熊尔山和蒲溪沟两个研究区的p H平均值显著升高了11.5%。与对照相比,土壤有机质、全氮、碱解氮、速效磷、速效钾、有效铁、有效锰和有效铜的含量都出现显著下降(P<0.05)。以干土计的细菌和古菌的基因拷贝数范围分别为2.42×10~7~5.81×10~7copies g~(-1)和1.77×10~6~5.66×10~6copies g~(-1),比对照降低了1~2个数量级。细菌数量高于古菌数量,而细菌和古菌均与土壤养分因子(土壤有机质,全氮,碱解氮,有效磷,速效钾,有效铁,有效锰)达到显著正相关水平,与土壤p H为极显著负相关,与有效铜含量没有显著相关关系。总之,地震破坏了土壤微生态环境,土壤细菌和古菌数量减少,土壤肥力降低,应进一步明确营养元素对土壤微生物影响的机理。 In order to explore the impact of earthquake disaster on forest soil fertility and microbial diversity, soil nutrient, bacteria and archaea community in different soil erosion degree in Li County, Sichuan Province were investigated. Two typical soil types, 7 Among them, one was the Cupressus chenginana forest, and one was affected by the earthquake at the Xiong’ershan survey site (mountainous cinnamon soil). Three affected forests were surveyed at the investigation point of Puxi ditch Mountain brown) Select one control and two affected forests. The results show that the average value of p H increased significantly by 11.5% in both Xiong’er and Puxi ditch affected by the earthquake. Compared with the control, soil organic matter, total nitrogen, available nitrogen, available phosphorus, available potassium, available iron, available manganese and available copper all decreased significantly (P <0.05). The gene copy number of bacteria and archaea with dry soil ranges from 2.42 × 10 ~ 7 ~ 5.81 × 10 ~ 7copies g ~ (-1) and 1.77 × 10 ~ 6 ~ 5.66 × 10 ~ 6copies g ~ (-1) ), Lower than the control by 1 to 2 orders of magnitude. The number of bacteria was higher than that of archaea, while the bacteria and archaea all had a significant positive correlation with soil nutrient (soil organic matter, total nitrogen, available nitrogen, available phosphorus, available potassium, available iron and available manganese) H is extremely significant negative correlation, and effective copper content is not significantly correlated. In short, the earthquake destroyed the micro-ecological environment, reducing the number of soil bacteria and archaea, reducing soil fertility and further clarifying the mechanism by which nutrient elements affect soil microorganisms.
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