Expression of the intact C4 type pepc gene cloned from maize in transgenic winter wheat

来源 :中国科学通报：英文版 | 被引量 : 0次 | 上传用户：wwucg

【摘要】

：

Maize intact C4-pepc gene was amplified through LA-PCR and successfully sub-cloned into modified vector pGreen0029 to form a stable expression construct named a

【作者】

：

CHENXuqing ZHANGXiaodong LIANG

【机构】

：

BeijingAgro-BiotechnologyResearchCenter.BeijingAcademyofAgricultural&,ForestryScience

【出处】

：

中国科学通报：英文版

【发表日期】

：

2004年20期

【关键词】

：

BAC DNA RNA MV PCR SDS-PAGE PDS C_4 photosynthesis pathway pepc gene transgeni

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Maize intact C4-pepc gene was amplified through LA-PCR and successfully sub-cloned into modified vector pGreen0029 to form a stable expression construct named as pBAC214 (12 kb), which contains CaMV 35S promoter driven bar gene as selection marker. Compar

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Expression of the intact C4 type pepc gene cloned from maize in transgenic winter wheat

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