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目的:测定山西产金莲花总黄酮及3种黄酮苷的含量。方法:紫外分光光度法测定金莲花总黄酮的含量,检测波长500nm。高效液相色谱法测定金莲花3种黄酮苷的含量,色谱柱为Alltima TM C18(4.6mm×250mm,5μm),流动相为乙腈(A)-水(B)梯度洗脱,流速为1.0m L/min,柱温为室温;检测波长360nm。结果:金莲花总黄酮在8.44-42.20μg/m L范围内线性关系良好,平均回收率为99.86%,RSD为1.42%;荭草苷、牡荆苷、槲皮素的进样量分别在0.40-4.00μg、0.036-0.360μg、0.032-0.320μg范围内呈良好的线性关系,平均回收率分别为99.65%、99.80%、99.13%,RSD分别为0.78%、1.20%、1.49%。结论:本方法简单准确,重复性好,适用于金莲花总黄酮及3种黄酮苷的含量测定。
Objective: To determine the content of total flavonoids and three kinds of flavonoid glycosides in Shanxi Golden Trollius. Methods: The content of total flavonoids in Trollius chinensis was determined by ultraviolet spectrophotometry. The detection wavelength was 500 nm. The contents of three flavonoid glycosides in Trollius chinensis L. were determined by HPLC. Alltima TM C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of a gradient of acetonitrile (A) -water (B) L / min, column temperature is room temperature; detection wavelength of 360nm. Results: The total flavonoids of Trollius chinensis had a good linearity in the range of 8.44-42.20 μg / mL with the average recovery of 99.86% and RSD of 1.42%. The injection volumes of eridium glycoside, vitexin and quercetin were 0.40 -4.00μg, 0.036-0.360μg, 0.032-0.320μg. The average recoveries were 99.65%, 99.80% and 99.13%, respectively. The RSDs were 0.78%, 1.20% and 1.49%, respectively. Conclusion: The method is simple, accurate and reproducible. It is suitable for the determination of total flavonoids and three flavonoid glycosides in Trollius chinensis.