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以马来丝虫感染期幼虫为靶抗原制备多株单克隆抗体（ＭｃＡｂ）从中筛选出１Ｇ１、１Ｈ１单克隆抗体，检测人工感染马来丝虫的中华按蚊。当中华按蚊吸食含马来微丝蚴的兔血后，分别解剖，同时以ＥＬＩＳＡ及Ｄｏｔ－ＥＬＩＳＡ检测饲养不同时间的中华按蚊。结果显示，饲养９ｄ的按蚊马来丝虫幼虫阳性率，人工解剖为８６．６％，ＥＬＩＳＡ和Ｄｏｔ－ＥＬＩＳＡ法分别为８２．２％和７７．８％。经统计学处理，人工解剖与单抗检测无显著性差异，其灵敏度为每只蚊０．５条幼虫。实验证明，此ＭｃＡｂ与牛腹腔指状丝虫、猪肺丝虫抗原无交叉反应，显示了一定的特异性。
In this study, monoclonal antibodies (McAbs) were prepared from larvae infected with Malayi as the target antigen. Monoclonal antibodies against 1G1 and 1H1 were screened out to detect Anopheles sinensis. When Chinese Anopheles mosquito inhalation of rabbit blood containing Malay microfilaria were dissected, respectively, while ELISA and Dot-ELISA detection of Anopheles sinensis at different times. The results showed that the positive rate of Malassezia larvae cultured for 9 days was 86.6% with artificial dissection and 82.2% with ELISA and Dot-ELISA respectively. After statistical analysis, there was no significant difference between artificial anatomy and monoclonal antibody detection, the sensitivity was 0.5 larvae per mosquito. Experiments show that this McAb and cattle abdominal filarial filarial filarial filarial antigen cross-reaction, showed a certain specificity.