目的　探讨肺癌发生的分子生物学机制 ;方法　采用逆转录 -巢式聚合酶链反应 (reverse- tape polymerase chainreaction)的方法对 4 2例肺癌及 10例正常肺组织中的 FHIT(Fragile histidine triad)基因的缺失情况进行检测 ,并用 PCR技术检测了肺癌组织中人乳头状瘤病毒 (human papilloma virus,HPV)的 DNA片段 ;结果　 6 6 .7% (2 8/ 4 3)肺癌组织中检测到 FHIT基因的缺失 ,而正常组织中未检测到 FHIT基因的缺失 ,二者差别有显著意义 (p<0 .0 1)。 4 2例肺癌组织中有 8例检测到 HPV的片段 ,阳性率为 19% (8/ 4 2 ) ,正常组织中未检测到 HPV的片段 ,且在 8例 HPV阳性的标本中均有 FHIT基因缺失。结论　 FHIT基因的缺失在肺癌的发生中起一定的作用 ;可与肺癌的不同病理分型、分化程度及临床分期无关 ;但与吸烟有一定的相关性 ;同时 HPV的感染与肺癌的发生有一定关系 ,且与 FHIT基因缺失呈正相关 Objective To explore the molecular biological mechanism of lung cancer development.Methods The reverse-nested polymerase chain reaction (reverse-tapper polymerase chain reaction) method was used to detect the FHIT (Fragile histidine triad) gene in 42 lung cancer patients and 10 normal lung tissues. The deletions were detected and DNA fragments of human papilloma virus (HPV) in lung cancer tissues were detected by PCR; the results showed that FHIT gene was detected in 66.7% (28/43) lung cancer tissues. The absence of FHIT was not detected in normal tissue, and there was a significant difference between the two (p<0.01). HPV fragments were detected in 8 out of 42 lung cancer tissues, with a positive rate of 19% (8/42). HPV fragments were not detected in normal tissues, and FHIT genes were found in 8 HPV-positive specimens. Missing. Conclusion The deletion of FHIT gene may play a role in the development of lung cancer. It may not be associated with different pathological types, differentiation and clinical stages of lung cancer. However, it has a certain correlation with smoking. At the same time, HPV infection is associated with the occurrence of lung cancer. Relationship, and positive correlation with FHIT gene deletion