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我们研究了刀豆素A(ConA)在体外诱导小鼠脾脏抑制性细胞所需的最适当剂量,发现约0.78μg/ml或50pμg/ml的浓度对大多数小鼠能诱导出以抑制性功能为主的细胞,这些细胞能抑制正常淋巴细胞(应答细胞)对促有丝分裂原的增生反应。6.25～12.50μg/ml的ConA所诱导的脾细胞能增强应答细胞对促有丝分裂原的增生反应。未经ConA诱导的脾细胞(对照细胞)可抑制或增强应答细胞对促有丝分裂原的增生反应。通常检测抑制性功能是应用相对抑制率来表示,但是它不能真实地反映ConA诱导细胞的功能。我们建议增加绝对抑制率的指标,因它能反映ConA诱导细胞的真实功能。这两种表示方法可以相互补充。
We investigated the most appropriate dose of concanavalin A (ConA) required to induce mouse spleen inhibitory cells in vitro and found that concentrations of about 0.78 μg / ml or 50 pg / ml induced in most mice an inhibitory function Predominant cells that inhibit the proliferative response of mitogen to normal lymphocytes (responding cells). ConA-induced splenocytes from 6.25 to 12.50 μg / ml potentiated the proliferative response of response cells to mitogens. Splenocytes (control cells) that are not induced by ConA can inhibit or enhance the proliferative response of the responding cells to mitogens. Usually the detection of inhibitory function is expressed by relative inhibition rate, but it can not truly reflect ConA-induced cell function. We suggest increasing the absolute inhibition rate as it reflects the true function of ConA-induced cells. These two representations can complement each other.