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目的:观察百草枯中毒大鼠血浆和肺组织匀浆中的内皮素-1的动态变化,探讨大鼠PQ中毒肺损伤的可能机制。方法:将雄性Wistar大鼠随机分成百草枯中毒组和生理盐水对照组,分别给予16 mg/kg百草枯或等体积生理盐水腹腔内注射,并在给药后6、12、24和72 h处死大鼠,观察其一般表现、肺的病理变化。测定肺系数、肺组织匀浆和血浆中内皮素-1的含量。结果:百草枯中毒后大鼠出现呼吸困难,活动能力下降,肺系数增加,并随时间变化,光镜下表现为肺出血、水肿等不同程度的肺损伤改变。血浆和组织匀浆中内皮素-1在中毒后与对照组大鼠比较明显升高,并随时间变化(P<0.01)。结论:在百草枯中毒肺损伤中,内皮素-1可能发挥了重要作用。
Objective: To observe the dynamic changes of endothelin - 1 in plasma and lung homogenates of paraquat poisoning rats, and to explore the possible mechanism of lung injury in rats with PQ poisoning. Methods: Male Wistar rats were randomly divided into paraquat poisoning group and saline control group, and were given intraperitoneal injection of 16 mg / kg paraquat or equal volume of saline, respectively, and were sacrificed at 6, 12, 24 and 72 h Rats, observe its general performance, pathological changes of the lungs. The lung coefficient, lung homogenate and plasma levels of endothelin-1 were measured. Results: After paraquat poisoning, rats developed dyspnea, decreased activity, increased lung coefficient, and changed with time. Under the light microscope, they showed different degrees of lung injury such as pulmonary hemorrhage and edema. Endothelin-1 in plasma and tissue homogenate were significantly increased compared with control group after poisoning, and changed with time (P <0.01). Conclusion: Endothelin-1 may play an important role in paraquat-induced lung injury.