在毛细管电泳仪上对12种β-受体阻滞剂和类似物的光学对映体进行了分离，研究了它们的拆分条件。使用0.1 mol/L, pH 3.0的磷酸-三乙醇胺缓冲液，20 kV电压，12～36 mmol/L羟丙基-β-环糊精作为手性识别试剂。其中11 种β-受体阻滞剂及其类似物以及苯乙醇胺、胺酵素在50 cm普通毛细管上获得了较好的手性分离效果，普罗勒沙的对映体在本条件下未获分离。同时发现，芳香环上取代基位置对迁移时间的影响有一定规律。“,”Chiral separation conditions of 12 kinds of β-blockers and β-blocker analogues were investigated by capillary electrophoresis. 11 of them, phenylethnolamine and clenbuterol were separated successfully under optim um conditions: 0.1 mol/L, pH 3.0 phosphate-trihydroxyethylamine buffer and proper concentration of hydroxypropyl-β-cyclodextrin (12～36　mmol/L) at 20 　kV voltage in 50　cm unmodified capillaries, but enantiomers of pronethalol was not separated. It was also found that the position of substituting group took effect on migration time.