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以黄苗榆烟草为试材、摩擦接种法接种烟草花叶病毒 ,比色法测定了几种防御酶活性 ,研究壳寡糖对烟草 烟草花叶病毒这一互作系统激发产生的一系列抗病生理生化反应的可能性。试验结果初步表明 :壳寡糖处理可导致烟草叶片PO、CAT、PPO、PAL和 β 1 3葡聚糖酶活性不同程度地提高。PO同工酶酶谱分析得知喷药后接毒处理可看到 7条酶带且谱带色浓 ,酶活性强。由此初步认为壳寡糖诱导抗病毒机制与提高PO活性及其增多同工酶酶谱的酶带有关 ;分析SOD、CAT和PPO三种酶似乎与壳寡糖诱导抗病毒机制相关不大或无相关性 ;单因素的喷药和接毒多可导致几种防御酶活性的提高 ,尤其是PAL和β 1 3葡聚糖酶活性提高明显 ,但喷药后接毒的酶活变化及升高幅度似乎与两单因素的加权效应无关
In order to study the effects of chitooligosaccharides on the tobacco mosaic virus, a series of disease resistance physiology Biochemical reaction possibilities. The results showed that the chitooligosaccharides treatment led to different degrees of PO, CAT, PPO, PAL and β 1 3 glucanase activities in tobacco leaves. PO isozyme zymogram analysis showed that after spraying the drug treatment can be seen seven bands and the band color strength, strong enzyme activity. Therefore, chitooligosaccharides-induced antiviral mechanism was suggested to be related to the increase of PO activity and the increase of enzyme zymogram bands. The analysis of SOD, CAT and PPO enzymes seemed to be not related to the chitooligosaccharide-induced antiviral mechanism or No correlation; single-factor spraying and poisoning can lead to an increase in the activity of several defense enzymes, especially PAL and β 1 3 glucanase activity increased significantly, but after spraying the drug-induced changes in enzyme activity and l The high amplitude seems to have nothing to do with the weighted effects of two single factors